Premium
Monitoring Combretastatin A4–induced tumor hypoxia and hemodynamic changes using endogenous MR contrast and DCE‐MRI
Author(s) -
Colliez Florence,
Fruytier AnneCatherine,
Magat Julie,
Neveu MarieAline,
Cani Patrice D.,
Gallez Bernard,
Jordan Bénédicte F.
Publication year - 2016
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25642
Subject(s) - hypoxia (environmental) , hemodynamics , magnetic resonance imaging , medicine , combretastatin , contrast (vision) , tumor hypoxia , endogeny , nuclear medicine , radiology , chemistry , cardiology , radiation therapy , oxygen , computer science , biology , organic chemistry , microtubule , artificial intelligence , tubulin , microbiology and biotechnology
Purpose To benchmark MOBILE (Mapping of Oxygen By Imaging Lipid relaxation Enhancement), a recent noninvasive MR method of mapping changes in tumor hypoxia, electron paramagnetic resonance (EPR) oximetry, and dynamic contrast‐enhanced MRI (DCE‐MRI) as biomarkers of changes in tumor hemodynamics induced by the antivascular agent combretastatin A4 (CA4). Methods NT2 and MDA‐MB‐231 mammary tumors were implanted subcutaneously in FVB/N and nude NMRI mice. Mice received 100 mg/kg of CA4 intraperitoneally 3 hr before imaging. The MOBILE sequence (assessing R1 of lipids) and the DCE sequence (assessing K trans hemodynamic parameter), were assessed on different cohorts. pO 2 changes were confirmed on matching tumors using EPR oximetry consecutive to the MOBILE sequence. Changes in tumor vasculature were assessed using immunohistology consecutive to DCE‐MRI studies. Results Administration of CA4 induced a significant decrease in lipids R 1 ( P = 0.0273) on pooled tumor models and a reduction in tumor pO 2 measured by EPR oximetry. DCE‐MRI also exhibited a significant drop of K trans ( P < 0.01) that was confirmed by immunohistology. Conclusion MOBILE was identified as a marker to follow a decrease in oxygenation induced by CA4. However, DCE‐MRI showed a higher dynamic range to follow changes in tumor hemodynamics induced by CA4. Magn Reson Med 75:866–872, 2016. © 2015 Wiley Periodicals, Inc.