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Feasibility of mapping unidirectional Pi‐to‐ATP fluxes in muscles of the lower leg at 7.0 Tesla
Author(s) -
Parasoglou Prodromos,
Xia Ding,
Regatte Ravinder R.
Publication year - 2015
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25388
Subject(s) - pi , chemistry , calf muscle , leg muscle , kinetics , nuclear magnetic resonance , soleus muscle , anatomy , skeletal muscle , physics , medicine , biochemistry , quantum mechanics , physical medicine and rehabilitation
Purpose To assess the feasibility of mapping the kinetics and unidirectional fluxes of inorganic phosphate (Pi) to adenosine triphosphate (ATP) reactions in the entire volume of the lower leg muscles using a three‐dimensional saturation transfer (ST) phosphorus ( 31 P) imaging sequence. Theory and Methods We imaged the lower leg muscles of five healthy subjects at 7.0 Tesla. The total experimental time was 45 min. We quantified muscle‐specific forward reaction rate constants ( k ′ f ) and metabolic fluxes ( V f ) of the Pi‐to‐ATP reaction in the tibialis anterior, the gastrocnemius, and the soleus. Results In the tibialis anterior, k ′ f and V f were 0.11 s −1 ± 0.03 (mean ± standard deviation) and 0.34 mM s −1 ± 0.10, respectively. In the gastrocnemius, k ′ f was 0.11 s −1 ± 0.04 and V f was 0.37 mM s −1 ± 0.11, while in the soleus muscle k ′ f was 0.10 s −1 ± 0.02 and V f was 0.36 mM s −1 ± 0.14. Conclusion Our results suggest that mapping the kinetics and unidirectional fluxes from Pi‐to‐ATP in both the anterior and posterior muscles of the lower leg is feasible at ultra‐high field and may provide useful insights for the study of insulin resistance, diabetes and aging. Magn Reson Med 74:225–230, 2015. © 2014 Wiley Periodicals, Inc.