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In vivo proton T 1 relaxation times of mouse myocardial metabolites at 9.4 T
Author(s) -
Bakermans Adrianus J.,
Abdurrachim Desiree,
Geraedts Tom R.,
Houten Sander M.,
Nicolay Klaas,
Prompers Jeanine J.
Publication year - 2015
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25340
Subject(s) - in vivo , chemistry , nuclear magnetic resonance , metabolite , ex vivo , taurine , nuclear magnetic resonance spectroscopy , creatine , proton , triglyceride , relaxation (psychology) , analytical chemistry (journal) , cholesterol , stereochemistry , medicine , chromatography , biochemistry , amino acid , biology , in vitro , physics , microbiology and biotechnology , quantum mechanics
Purpose Proton magnetic resonance spectroscopy ( 1 H‐MRS) for quantitative in vivo assessment of mouse myocardial metabolism requires accurate acquisition timing to minimize motion artifacts and corrections for T 1 ‐dependent partial saturation effects. In this study, mouse myocardial water and metabolite T 1 relaxation time constants were quantified. Methods Cardiac‐triggered and respiratory‐gated PRESS‐localized 1 H‐MRS was employed at 9.4 T to acquire signal from a 4‐µL voxel in the septum of healthy mice ( n = 10) while maintaining a steady state of magnetization using dummy scans during respiratory gates. Signal stability was assessed via standard deviations (SD) of zero‐order phases and amplitudes of water spectra. Saturation‐recovery experiments were performed to determine T 1 values. Results Phase SD did not vary for different repetition times (TR), and was 13.1° ± 4.5°. Maximal amplitude SD was 14.2% ± 5.1% at TR = 500 ms. Myocardial T 1 values (mean ± SD) were quantified for water (1.71 ± 0.25 s), taurine (2.18 ± 0.62 s), trimethylamine from choline‐containing compounds and carnitine (1.67 ± 0.25 s), creatine‐methyl (1.34 ± 0.19 s), triglyceride‐methylene (0.60 ± 0.15 s), and triglyceride‐methyl (0.90 ± 0.17 s) protons. Conclusion This work provides in vivo quantifications of proton T 1 values for mouse myocardial water and metabolites at 9.4 T. Magn Reson Med 73:2069–2074, 2015. © 2014 Wiley Periodicals, Inc.