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Multiparametric optical and MR imaging demonstrate inhibition of tumor angiogenesis natural history by mural cell therapy
Author(s) -
Faye Nathalie,
Clément Olivier,
Balvay Daniel,
Fitoussi Victor,
Pidial Laetitia,
Sandoval Federico,
Autret Gwennhael,
Silvestre Jean Sébastien,
Dean Carole Leré,
Alison Marianne,
Fournier Laure S.
Publication year - 2014
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24970
Subject(s) - carbogen , perfusion , microcirculation , vascular permeability , angiogenesis , chemistry , in vivo , mural cell , photodynamic therapy , intravoxel incoherent motion , effective diffusion coefficient , nuclear medicine , confocal , saline , neovascularization , magnetic resonance imaging , pathology , medicine , oxygen , endothelial stem cell , cancer research , in vitro , radiology , biology , biochemistry , geometry , microbiology and biotechnology , mathematics , organic chemistry
Purpose To determine whether functional imaging using MRI and fibered confocal fluorescence microscopy (FCFM) could be used to monitor cell therapy by mural progenitor cells (MPC). Methods Fifty mice bearing TC1 murine xenograft tumors were allocated into: control (n = 17), sham (phosphate buffer saline, n = 16), and MPC‐treated (MPC, n = 17) groups. MRI was performed before (D 0 ) and 7 days (D 7 ) after injection measuring tumor size, R 2 * under air, oxygen, and carbogen using blood oxygen level dependent (BOLD) and f (fraction linked to microcirculation), D* (perfusion related coefficient) and Dr (restricted diffusion coefficient) using diffusion‐weighted sequences based on the IVIM (intravoxel incoherent motion) method. FCFM was performed at D 7 measuring “index leakage” (capillary permeability). Results Tumor growth was significantly slowed down in the MPC‐treated animals ( P = 0.002) on D 7 . R 2 * air significantly decreased in controls between D 0 and D 7 ( P = 0.03), reflecting a decrease in tumor oxygenation. ΔR 2 * O2CO2 significantly increased in controls between D 0 and D 7 ( P = 0.01) reflecting loss of vessel response to carbogen. D* significantly decreased in controls between D 0 and D 7 ( P = 0.03). Finally, “index leakage” was lower in the MPC‐treated tumors ( P = 0,009). Conclusion Treatment by MPC resulted in slowing down of tumor growth, capillary permeability decrease, and stabilization of tumor angiogenesis. Magn Reson Med 72:841–849, 2014. © 2013 Wiley Periodicals, Inc.