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Reproducibility study for free‐breathing measurements of pyruvate metabolism using hyperpolarized 13 C in the heart
Author(s) -
Lau Angus Z.,
Chen Albert P.,
Barry Jennifer,
Graham John J.,
DominguezViqueira William,
Ghugre Nilesh R.,
Wright Graham A.,
Cunningham Charles H.
Publication year - 2013
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24752
Subject(s) - pyruvate dehydrogenase complex , in vivo , lactate dehydrogenase a , reproducibility , chemistry , lactate dehydrogenase , respiration , metabolism , bicarbonate , medicine , nuclear magnetic resonance , nuclear medicine , biochemistry , biology , enzyme , chromatography , anatomy , physics , microbiology and biotechnology
Spatially resolved images of hyperpolarized 13 C substrates and their downstream products provide insight into real‐time metabolic processes occurring in vivo. Recently, hyperpolarized 13 C pyruvate has been used to characterize in vivo cardiac metabolism in the rat and pig, but accurate and reproducible measurements remain challenging due to the limited period available for imaging as well as physiological motion. In this article, time‐resolved cardiac‐ and respiratory‐gated images of [1‐ 13 C] pyruvate, [1‐ 13 C] lactate, and 13 C bicarbonate in the heart are acquired without the need for a breathhold. The robustness of these free‐breathing measurements is demonstrated using the time‐resolved data to produce a normalized metric of pyruvate dehydrogenase and lactate dehydrogenase activity in the heart. The values obtained are reproducible in a controlled metabolic state. In a 60‐min ischemia/reperfusion model, significant differences in hyperpolarized bicarbonate and lactate, normalized using the left ventricular pyruvate signal, were detected between scans performed at baseline and 45 min after reperfusion. The sequence is anticipated to improve quantitative measurements of cardiac metabolism, leading to feasible validation studies using fewer subjects, and potentially improved diagnosis, serial monitoring, and treatment of cardiac disease in patients. © 2013 Wiley Periodicals, Inc.