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Collagen Composition and Content‐Dependent Contrast in Porcine Annulus Fibrosus Achieved by Using Double Quantum and Magnetization Transfer Filtered UTE MRI
Author(s) -
Eliav Uzi,
Komlosh Michal E.,
Basser Peter J.,
Navon Gil
Publication year - 2014
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24662
Subject(s) - annulus (botany) , magnetization transfer , nuclear magnetic resonance , chemistry , magnetization , intervertebral disc , pulse sequence , materials science , analytical chemistry (journal) , anatomy , magnetic field , magnetic resonance imaging , physics , composite material , biology , medicine , chromatography , quantum mechanics , radiology
Purpose To test the potential of combining double quantum and magnetization transfer filtered ultra‐short echo time (DQF‐MT‐UTE) MRI to obtain information about the macromolecular composition and characteristics of connective tissues. Methods A DQF‐MT‐UTE pulse sequence was implemented on a 14.1 T AVANCE III Bruker spectrometer equipped with a Bruker micro2.5‐imaging gradient system to obtain images of porcine annulus fibrosus. Results The DQF‐MT‐UTE MRI of the annulus fibrosus of porcine intervertebral disc, where the creation time of the double quantum coherence filtering (DQF) was on a time scale appropriate for excitation of macromolecules, showed stronger signal from the outer layers of the disc than from the inner layers closer to the nucleus pulposus. Similarly, spectroscopic studies showed the same trend in the efficiency of the magnetization transfer (MT) from collagen to water. Conclusion DQF‐MT filtered UTE MRI of the annulus fibrosus provides new contrast parameters that depend on the concentration of the collagen and on the rate and efficiency of MT of its protons to water. The latter parameters appear to be different for collagen types I and II in the annulus fibrosus. Magn Reson Med 71:388–393, 2014. © 2013 Wiley Periodicals, Inc.