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Quantitative measurement of cancer metabolism using stimulated echo hyperpolarized carbon‐13 MRS
Author(s) -
Swisher Christine Leon,
Larson Peder E. Z.,
Kruttwig Klaus,
Kerr Adam B.,
Hu Simon,
Bok Robert A.,
Goga Andrei,
Pauly John M.,
Nelson Sarah J.,
Kurhanewicz John,
Vigneron Daniel B.
Publication year - 2014
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24634
Subject(s) - chemistry , in vivo , nuclear magnetic resonance , relaxation (psychology) , nuclear magnetic resonance spectroscopy , hyperpolarization (physics) , analytical chemistry (journal) , biological system , chromatography , physics , stereochemistry , psychology , social psychology , microbiology and biotechnology , biology
Purpose Magnetic resonance spectroscopy of hyperpolarized substrates allows for the observation of label exchange catalyzed by enzymes providing a powerful tool to investigate tissue metabolism and potentially kinetics in vivo. However, the accuracy of current methods to calculate kinetic parameters has been limited by T 1 relaxation effects, extracellular signal contributions, and reduced precision at lower signal‐to‐noise ratio. Theory and Methods To address these challenges, we investigated a new modeling technique using metabolic activity decomposition‐stimulated echo acquisition mode. The metabolic activity decomposition‐stimulated echo acquisition mode technique separates exchanging from nonexchanging metabolites providing twice the information as conventional techniques. Results This allowed for accurate measurements of rates of conversion and of multiple T 1 values simultaneously using a single acquisition. Conclusion The additional measurement of T 1 values for the reaction metabolites provides further biological information about the cellular environment of the metabolites. The new technique was investigated through simulations and in vivo studies of transgenic mouse models of cancer demonstrating improved assessments of kinetic rate constants and new T 1 relaxation value measurements for hyperpolarized 13 C‐pyruvate, 13 C‐lactate, and 13 C‐alanine. Magn Reson Med 71:1–11, 2014. © 2013 Wiley Periodicals, Inc.