Premium
Evaluation of heterogeneous metabolic profile in an orthotopic human glioblastoma xenograft model using compressed sensing hyperpolarized 3D 13 C magnetic resonance spectroscopic imaging
Author(s) -
Park Ilwoo,
Hu Simon,
Bok Robert,
Ozawa Tomoko,
Ito Motokazu,
Mukherjee Joydeep,
Phillips Joanna J.,
James C. David,
Pieper Russell O.,
Ronen Sabrina M.,
Vigneron Daniel B.,
Nelson Sarah J.
Publication year - 2013
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24434
Subject(s) - magnetic resonance imaging , nuclear magnetic resonance , imaging phantom , chemistry , magnetic resonance spectroscopic imaging , compressed sensing , metabolite , molecular imaging , nuclear medicine , pathology , biomedical engineering , in vivo , medicine , biology , radiology , physics , biochemistry , microbiology and biotechnology , artificial intelligence , computer science
High resolution compressed sensing hyperpolarized 13 C magnetic resonance spectroscopic imaging was applied in orthotopic human glioblastoma xenografts for quantitative assessment of spatial variations in 13 C metabolic profiles and comparison with histopathology. A new compressed sensing sampling design with a factor of 3.72 acceleration was implemented to enable a factor of 4 increase in spatial resolution. Compressed sensing 3D 13 C magnetic resonance spectroscopic imaging data were acquired from a phantom and 10 tumor‐bearing rats following injection of hyperpolarized [1‐ 13 C]‐pyruvate using a 3T scanner. The 13 C metabolic profiles were compared with hematoxylin and eosin staining and carbonic anhydrase 9 staining. The high‐resolution compressed sensing 13 C magnetic resonance spectroscopic imaging data enabled the differentiation of distinct 13 C metabolite patterns within abnormal tissues with high specificity in similar scan times compared to the fully sampled method. The results from pathology confirmed the different characteristics of 13 C metabolic profiles between viable, non‐necrotic, nonhypoxic tumor, and necrotic, hypoxic tissue. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.