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In vivo MRI visualization of different cell populations labeled with PARACEST agents
Author(s) -
Ferrauto Giuseppe,
Castelli Daniela Delli,
Terreno Enzo,
Aime Silvio
Publication year - 2013
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24411
Subject(s) - in vivo , paramagnetism , saturation (graph theory) , chemistry , cell , nuclear magnetic resonance , in vitro , relaxation (psychology) , biophysics , biochemistry , biology , physics , microbiology and biotechnology , mathematics , combinatorics , quantum mechanics , neuroscience
Conventional T 1 ‐ or T 2 ‐MRI contrast agents do not allow to track the distribution of different cell populations simultaneously because the effects of relaxation enhancers are additive. Herein, it is shown that paramagnetic chemical exchange saturation transfer agents offer the opportunity to visualize different cell populations in vitro and in vivo by 1 H‐MRI. Yb‐ and Eu‐HPDO3A complexes have been used to label murine macrophages (J774.A1) and melanoma cells (B16‐F10), respectively. By selective irradiation of the highly‐shifted OH resonances of the two chemical exchange saturation transfer agents, it has been shown that tracking of the two cell types is possible. These PARAmagnetic Chemical Exchange Saturation Transfer agents have a tremendous potential for clinical translation as they share the same stability and in vivo pharmacokinetic properties of Gd‐HPDO3A (ProHance ® ), which is a widely used clinically approved MRI agent. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.