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HyperCEST detection of a 129 Xe‐based contrast agent composed of cryptophane‐A molecular cages on a bacteriophage scaffold
Author(s) -
Stevens Todd K.,
Palaniappan Krishnan K.,
Ramirez R. Matthew,
Francis Matthew B.,
Wemmer David E.,
Pines Alex
Publication year - 2013
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24371
Subject(s) - xenon , chemistry , bacteriophage , saturation (graph theory) , nuclear magnetic resonance , organic chemistry , physics , biochemistry , mathematics , escherichia coli , combinatorics , gene
A hyperpolarized 129 Xe contrast agent composed of many cryptophane‐A molecular cages assembled on an M13 bacteriophage has been demonstrated. Saturation of xenon bound in the large number of cryptophane cages is transferred to the pool of aqueous‐solvated xenon via chemical exchange, resulting in efficient generation of hyperCEST contrast. No significant loss of contrast per cryptophane cage was observed for the multivalent phage when compared with unscaffolded cryptophane. Detection of this phage‐based hyperCEST agent is reported at concentrations as low as 230 fM, representing the current lower limit for NMR/MRI‐based contrast agents. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.