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Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1‐ 13 C]pyruvate
Author(s) -
Park Jae Mo,
Josan Sonal,
Jang Taichang,
Merchant Milton,
Yen YiFen,
Hurd Ralph E.,
Recht Lawrence,
Spielman Daniel M.,
Mayer Dirk
Publication year - 2012
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24181
Subject(s) - glioma , chemistry , metabolite , kinetics , nuclear magnetic resonance , reaction rate constant , nuclear medicine , medicine , biochemistry , cancer research , physics , quantum mechanics
In addition to an increased lactate‐to‐pyruvate ratio, altered metabolism of a malignant glioma can be further characterized by its kinetics. Spatially resolved dynamic data of pyruvate and lactate from C6‐implanted female Sprague–Dawley rat brain were acquired using a spiral chemical shift imaging sequence after a bolus injection of a hyperpolarized [1‐ 13 C]pyruvate. Apparent rate constants for the conversion of pyruvate to lactate in three different regions (glioma, normal appearing brain, and vasculature) were estimated based on a two‐site exchange model. The apparent conversion rate constant was 0.018 ± 0.004 s −1 (mean ± standard deviation, n = 6) for glioma, 0.009 ± 0.003 s −1 for normal brain, and 0.005 ± 0.001 s −1 for vasculature, whereas the lactate‐to‐pyruvate ratio, the metabolic marker used to date to identify tumor regions, was 0.36 ± 0.07 (mean ± SD), 0.24 ± 0.07, and 0.12 ± 0.02 for glioma, normal brain, and vasculature, respectively. The data suggest that the apparent conversion rate better differentiate glioma from normal brain ( P = 0.001, n = 6) than the lactate‐to‐pyruvate ratio ( P = 0.02). Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.