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Microscopic diffusion anisotropy in formalin fixed prostate tissue: Preliminary findings
Author(s) -
Bourne Roger M.,
Kurniawan Nyoman,
Cowin Gary,
Sved Paul,
Watson Geoffrey
Publication year - 2012
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.24179
Subject(s) - anisotropy , diffusion mri , fractional anisotropy , nuclear magnetic resonance , prostate , isotropy , materials science , stromal cell , voxel , diffusion , chemistry , biomedical engineering , pathology , medicine , magnetic resonance imaging , physics , optics , radiology , cancer , thermodynamics
Diffusion tensor microimaging at 16.4 T with 40 μm isotropic voxels was used to investigate anisotropic water diffusion in prostate tissue at spatial resolution approaching the cellular scale. Nine normal glandular tissue samples were collected from the peripheral zone of six formalin fixed radical prostatectomy specimens. Fibromuscular stromal tissue exhibited microscopic diffusion anisotropy (mean fractional anisotropy range 0.47–0.66) significantly higher ( P < 0.01, Student's t ‐test) than in epithelium‐containing voxels (mean fractional anisotropy range 0.31–0.54) in six of the seven normal tissue samples in which both compartments could be measured. Fiber tracking demonstrated principle stromal fiber directions consistent with myocyte orientation seen on light microscopy of the same sample. Diffusion tensor microimaging may be valuable for investigation of variable results from attempts to measure diffusion anisotropy in the prostate in vivo. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.