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Multitissue assessment of in vivo postprandial intracellular lipid partitioning in rats using localized 1 H‐[ 13 C] magnetic resonance spectroscopy
Author(s) -
Jonkers Richard A. M.,
Geraedts Tom R.,
van Loon Luc J. C.,
Nicolay Klaas,
Prompers Jeanine J.
Publication year - 2012
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.23321
Subject(s) - in vivo , postprandial , chemistry , skeletal muscle , lipid metabolism , nuclear magnetic resonance spectroscopy , lipid accumulation , endocrinology , intracellular , medicine , biochemistry , biology , insulin , stereochemistry , microbiology and biotechnology
Excess accumulation of lipids in nonadipose tissues such as skeletal muscle and liver has been implicated in the development of obesity‐related disorders, but the cause of this ectopic lipid overload remains unknown. The aim of this study was to determine in vivo postprandial lipid partitioning in rat skeletal muscle and liver, using localized 1 H‐[ 13 C] magnetic resonance spectroscopy in combination with the oral administration of 13 C‐labeled lipids. Six rats were measured at baseline and 5 and 24 h after administration of 400 mg [U‐ 13 C]‐labeled algal lipids. Five hours after administration, fractional 13 C enrichments of the lipid pools in muscle and liver were increased 3.9‐fold and 4.6‐fold ( P < 0.05), respectively, indicating that part of the ingested lipids had been taken up by muscle and liver tissue. At 24 h, fractional 13 C enrichments of muscle and liver lipids were decreased 1.6‐fold and 2.2‐fold ( P < 0.05), respectively, compared with the 5 h values. This can be interpreted as a depletion of 13 C‐labeled lipids from the intracellular lipid pools as a consequence of lipid turnover. In conclusion, the novel application of 1 H‐[ 13 C] magnetic resonance spectroscopy in combination with the oral administration of 13 C‐labeled lipids is applicable for the longitudinal assessment of in vivo lipid partitioning between multiple tissues. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.