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T 2 ‐weighted MRI of post‐infarct myocardial edema in mice
Author(s) -
Beyers Ronald J.,
Smith R. Scott,
Xu Yaqin,
Piras Bryan A.,
Salerno Michael,
Berr Stuart S.,
Meyer Craig H.,
Kramer Christopher M.,
French Brent A.,
Epstein Frederick H.
Publication year - 2012
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22975
Subject(s) - medicine , magnetic resonance imaging , myocardial infarction , cardiology , nuclear medicine , ischemia , edema , infarction , in vivo , radiology , microbiology and biotechnology , biology
Abstract T 2 ‐weighted, cardiac magnetic resonance imaging ( T 2 w CMR) can be used to noninvasively detect and quantify the edematous region that corresponds to the area at risk (AAR) following myocardial infarction (MI). Previously, CMR has been used to examine structure and function in mice, expediting the study of genetic manipulations. To date, CMR has not been applied to imaging of post‐MI AAR in mice. We developed a whole‐heart, T 2 w CMR sequence to quantify the AAR in mouse models of ischemia and infarction. The Δ B 0 and Δ B 1 environment around the mouse heart at 7 T were measured, and a T 2 ‐preparation sequence suitable for these conditions was developed. Both in vivo T 2 w and late gadolinium enhanced CMR were performed in mice after 20‐min coronary occlusions, resulting in measurements of AAR size of 32.5 ± 3.1 (mean ± SEM)% left ventricular mass, and MI size of 50.1 ± 6.4% AAR size. Excellent interobserver agreement and agreement with histology were also found. This T 2 w imaging method for mice may allow for future investigations of genetic manipulations and novel therapies affecting the AAR and salvaged myocardium following reperfused MI. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.