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Enhanced neurochemical profile of the rat brain using in vivo 1 H NMR spectroscopy at 16.4 T
Author(s) -
Hong SungTak,
Balla Dávid Zsolt,
Shajan G.,
Choi Changho,
Uğurbil Kâmil,
Pohmann Rolf
Publication year - 2011
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22609
Subject(s) - in vivo , neurochemical , nuclear magnetic resonance , metabolite , nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , echo time , analytical chemistry (journal) , magnetic resonance imaging , chromatography , biochemistry , physics , biology , medicine , microbiology and biotechnology , radiology , quantum mechanics , endocrinology
Single voxel magnetic resonance spectroscopy with ultrashort echo time was implemented at 16.4 T to enhance the neurochemical profile of the rat brain in vivo. A TE of 1.7 msec was achieved by sequence optimization and by using short‐duration asymmetric pulses. Macromolecular signal components were parameterized individually and included in the quantitative analysis, replacing the use of a metabolite‐nulled spectrum. Because of the high spectral dispersion, several signals close to the water line could be detected, and adjacent peaks could be resolved. All 20 metabolites detected in this study were quantified with Cramér‐Rao lower bounds below 20%, implying reliable quantification accuracy. The signal of acetate was detected for the first time in rat brain in vivo with Cramér‐Rao lower bounds of 16% and a concentration of 0.52 μmol/g. The absolute concentrations of most metabolites showed close agreement with values previously measured using in vivo 1 H NMR spectroscopy and in vitro biochemical assay. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.