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Magnetosonoporation: Instant magnetic labeling of stem cells
Author(s) -
Qiu Bensheng,
Xie Daohai,
Walczak Piotr,
Li Xubin,
RuizCabello Jesus,
Minoshima Satoshi,
Bulte Jeff W.M.,
Yang Xiaoming
Publication year - 2010
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22348
Subject(s) - stem cell , in vivo , neural stem cell , cell , viability assay , magnetic resonance imaging , biomedical engineering , chemistry , biology , medicine , microbiology and biotechnology , radiology , biochemistry
Abstract The purpose of this study was to develop an instant MR cell labeling technique, called magnetosonoporation. First, a magnetosonoporation apparatus was successfully established for MR labeling of stem cells. Then, the safety of this new cell labeling approach was confirmed by evaluation of cell viability, proliferation, and differentiation of magnetosonoporation‐labeled and unlabeled C17.2 neural stem cells. Subsequently, the feasibility of using in vivo MRI to detect magnetosonoporation/Feridex‐labeled stem cells was validated in living animals and confirmed by histologic correlation. The magnetosonoporation technique is expected to be convenient, efficient, and safe for future clinical application of MRI‐guided cell therapies. Magn Reson Med 63:1437–1441, 2010. © 2010 Wiley‐Liss, Inc.