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Evaluation of magnetic resonance vessel size imaging by two‐photon laser scanning microscopy
Author(s) -
Douma Kim,
Oostendorp Marlies,
Slaaf Dick W.,
Post Mark J.,
Backes Walter H.,
van Zandvoort Marc A. M. J.
Publication year - 2010
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22248
Subject(s) - microvessel , magnetic resonance imaging , microcirculation , two photon excitation microscopy , blood vessel , biomedical engineering , blood volume , volume (thermodynamics) , radius , pathology , nuclear magnetic resonance , materials science , medicine , physics , radiology , computer science , immunohistochemistry , optics , quantum mechanics , psychiatry , cardiology , fluorescence , computer security
MR vessel size imaging (MR‐VSI) is increasingly applied to noninvasively assess microvascular properties of tumors and to evaluate tumor response to antiangiogenic treatment. MR‐VSI provides measures for the microvessel radius and fractional blood volume of tumor tissue. However, data have not yet been evaluated with three‐dimensional microscopy techniques. Therefore, three‐dimensional two‐photon laser scanning microscopy (TPLSM) was performed to assess microvascular radius and fractional vessel volume in tumor and muscle tissue. TPLSM data displayed a mazelike architecture of the tumor microvasculature and mainly parallel oriented muscle microvessels. For both MR‐VSI and TPLSM, a larger vessel radius and fractional blood volume were found in the tumor rim than in the core. The microvessel radius was approximately six times larger in tumor and muscle for MR‐VSI than for TPLSM. The tumor blood volume was 4‐fold lower with MR‐VSI than with TPLSM, whereas muscle blood volume was comparable for both techniques. Differences between the tumor rim, core, and muscle tissue showed similar trends for both MR‐VSI and TPLSM parameters. These results indicate that MR‐VSI does not provide absolute measures of microvascular morphology; however, it does reflect heterogeneity in microvascular morphology. Hence, MR‐VSI may be used to assess differences in microvascular morphology. Magn Reson Med 63:930–939, 2010. © 2010 Wiley‐Liss, Inc.