Premium
Slow magic‐angle coil spinning: A high‐sensitivity and high‐resolution NMR strategy for microscopic biological specimens
Author(s) -
Wong Alan,
Aguiar Pedro M.,
Sakellariou Dimitris
Publication year - 2010
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22231
Subject(s) - magic angle spinning , microcoil , spinning , magic (telescope) , magic angle , materials science , resolution (logic) , high resolution , electromagnetic coil , nuclear magnetic resonance , analytical chemistry (journal) , chemistry , physics , nuclear magnetic resonance spectroscopy , computer science , composite material , chromatography , remote sensing , quantum mechanics , artificial intelligence , geology
Abstract High‐resolution magic‐angle spinning has become a valuable tool to study biologic samples; however, there are only a few high‐resolution magic‐angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high‐resolution 1 H NMR spectroscopic approach using magic‐angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large‐volume commercial magic‐angle spinning rotor, where the microcoil offers incredible sensitivity and the large‐volume magic‐angle spinning rotor provides stable slow magic‐angle spinning. This approach provides high‐resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.