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Quantitative 19 F imaging of nmol‐level F‐nucleotides/‐sides from 5‐FU with T 2 mapping in mice at 9.4T
Author(s) -
Doi Yoshihiro,
Shimmura Toshiyuki,
Kuribayashi Hideto,
Tanaka Yoshitsugu,
Kanazawa Yoko
Publication year - 2009
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22075
Subject(s) - nucleotide , physics , chemistry , nuclear medicine , nuclear magnetic resonance , medicine , biochemistry , gene
A unique acquisition method is proposed for quantitative, high‐sensitivity 19 F MR spectroscopic imaging for the study of drug distribution aiming at nmol‐level metabolite information in mice. The use of fast spin echo (FSE) at 9.4T allowed us to obtain whole‐body images with minimal effect of magnetic susceptibility and to acquire several metabolite signals simultaneously by the method of interleaved multifrequency selection. Modified 2‐shot FSE was designed for simultaneous, high‐sensitivity 19 F imaging and T 2 mapping. A time course study including all the main metabolites at 10‐minute resolution was attained with an oral dose of 1–2 mmol 5‐fluorouracil (5‐FU) (130–260 mg)/kg in mice. With acquisition parameters optimized for in vivo T 2 of 40 ms, images of F‐nucleotides/‐sides, effective anabolites of the anticancer drug 5‐FU, were obtained at the level of 200 nmol in the tumor for all the mice studied with a linear correlation (R = 0.96) between image intensity and the quantity determined in the excised tissue. The method exhibits potential capability of molecular imaging with a variety of 19 F‐labeled compounds and drug evaluation. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.