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Manganese‐guided cellular MRI of human embryonic stem cell and human bone marrow stromal cell viability
Author(s) -
Yamada Mayumi,
Gurney Paul T.,
Chung Jaehoon,
Kundu Pratima,
Drukker Micha,
Smith Alan K.,
Weissman Irving L.,
Nishimura Dwight,
Robbins Robert C.,
Yang Phillip C.
Publication year - 2009
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22071
Subject(s) - viability assay , stromal cell , embryonic stem cell , bone marrow , bioluminescence imaging , in vivo , stem cell , chemistry , pathology , cell , cell culture , microbiology and biotechnology , biology , medicine , transfection , biochemistry , luciferase , genetics , gene
This study investigated the ability of MnCl 2 as a cellular MRI contrast agent to determine the in vitro viability of human embryonic stem cells (hESC) and human bone marrow stromal cells (hBMSC). Basic MRI parameters including T 1 and T 2 values of MnCl 2 ‐labeled hESC and hBMSC were measured and viability signal of manganese (Mn 2+ )‐labeled cells was validated. Furthermore, the biological activity of Ca 2+ ‐channels was modulated utilizing both Ca 2+ ‐channel agonist and antagonist to evaluate concomitant signal changes. Metabolic effects of MnCl 2 ‐labeling were also assessed using assays for cell viability, proliferation, and apoptosis. Finally, in vivo Mn 2+ ‐guided MRI of the transplanted hESC was successfully achieved and validated by bioluminescence imaging. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.
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