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Quantifying endogenous glucose production and contributing source fluxes from a single 2 H NMR spectrum
Author(s) -
Nunes Patricia M.,
Jones John G.
Publication year - 2009
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.22062
Subject(s) - chemistry , glycerol , glycogen , endogeny , plasma glucose , mole , pi , medicine , carbohydrate , endocrinology , carbohydrate metabolism , biochemistry , insulin , biology
Endogenous glucose production (EGP), gluconeogenic and glycogenolytic fluxes by analysis of a single 2 H‐NMR spectrum is demonstrated with 6‐hr and 24‐hr fasted rats. Animals were administered [1‐ 2 H, 1‐ 13 C]glucose, a novel tracer of glucose turnover, and 2 H 2 O. Plasma glucose enrichment from both tracers was quantified by 2 H‐NMR analysis of monoacetone glucose. The 6‐hr fasted group ( n = 7) had EGP rates of 95.6 ± 13.3 μmol/kg/min, where 56.2 ± 7.9 μmol/kg/min were derived from PEP; 12.1 ± 2.1 μmol/kg/min from glycerol, and 32.1 ± 4.9 μmol/kg/min from glycogen. The 24‐hr fasted group ( n = 7) had significantly lower EGP rates (52.8 ± 7.2 μmol/kg/min, P = 0.004 vs. 6 hr) mediated by a significantly reduced contribution from glycogen (4.7 ± 5.9 μmol/kg/min, P = 0.02 vs. 6 hr) while PEP and glycerol contributions were not significantly different (39.5 ± 3.9 and 8.5 ± 1.2 μmol/kg/min, respectively). These estimates agree with previous assays of EGP fluxes in fasted rats obtained by multinuclear NMR analyses of plasma glucose enrichment from 2 H 2 O and 13 C‐glucose tracers. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.