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Ischemia‐induced changes of intracellular water diffusion in rat glioma cell cultures
Author(s) -
Trouard Theodore P.,
Harkins Kevin D.,
Divijak Joseph L.,
Gillies Robert J.,
Galons JeanPhilippe
Publication year - 2008
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21616
Subject(s) - intracellular , extracellular , glioma , ischemia , effective diffusion coefficient , diffusion , chemistry , diffusion mri , cell , cell culture , biophysics , nuclear magnetic resonance , biomedical engineering , medicine , magnetic resonance imaging , biology , cancer research , biochemistry , cardiology , radiology , physics , genetics , thermodynamics
Diffusion‐weighted MRI is commonly used in the diagnosis and evaluation of ischemic stroke because of the rapid decrease observed in the apparent diffusion coefficient (ADC) of tissue water following ischemia. Although this observation has been clinically useful for many years, the biophysical mechanisms underlying the reduction of tissue ADC are still unknown. To help elucidate these mechanisms, we have employed a novel three‐dimensional (3D) hollow‐fiber bioreactor (HFBR) perfused cell culture system that enables cells to be grown to high density and studied via MRI and MRS. By infusing contrast media into the HFBR, signals from intracellular water and extracellular water are spectroscopically resolved and can be investigated individually. Diffusion measurements carried out on C6 glioma HFBR cell cultures indicate that ischemia‐induced cellular swelling results in an increase in the ADC of intracellular water from 0.35 μm 2 /ms to approximately 0.5 μm 2 /ms (diffusion time = 25 ms). Magn Reson Med 60:258–264, 2008. © 2008 Wiley‐Liss, Inc.