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In vivo quantification of T 1 , T 2 , and apparent diffusion coefficient in the mouse retina at 11.74T
Author(s) -
Chen Junjie,
Wang Qing,
Zhang Huiying,
Yang Xiaoxia,
Wang Jian,
Berkowitz Bruce A.,
Wickline Samuel A.,
Song ShengKwei
Publication year - 2008
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21570
Subject(s) - retina , choroid , retinal , optic nerve , effective diffusion coefficient , diffusion mri , outer nuclear layer , nuclear magnetic resonance , chemistry , anatomy , pathology , ophthalmology , biology , physics , magnetic resonance imaging , neuroscience , medicine , radiology
MRI has recently been used for noninvasive examination of retinal structure and function in rats and cats. However, the advantages of quantitative high‐resolution MRI of retina from mice have not yet been explored. In the present study, T 1 and T 2 relaxation time constants and the directional apparent diffusion coefficient (ADC) in the retina of C57/BL6 mice were measured. Three MR‐detected retina layers and a MR‐detected choroid layer were observed on both T 1 ‐ and T 2 ‐weighted images at an image resolution of 47 × 47 × 400 μm 3 . The significantly higher ADC parallel to than that perpendicular to the optic nerve in the MR‐detected outer retina layer at the central retina reflects the known cellular organization of the photoreceptor cells. This study establishes, for the first time, normative metrics of T 1 , T 2 , and ADC of the mouse retina. These MR parameters are expected to be useful in future evaluation of developmental and pathological alterations of retinal cell layers in mice. Magn Reson Med 59:731–738, 2008. © 2008 Wiley‐Liss, Inc.