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Localized sensitivity enhanced in vivo 13 C MRS to detect glucose metabolism in the mouse brain
Author(s) -
Nabuurs C.I.H.C.,
Klomp D.W.J.,
Veltien A.,
Kan H.E.,
Heerschap A.
Publication year - 2008
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21498
Subject(s) - in vivo , in vivo magnetic resonance spectroscopy , glutamine , chemistry , glutamatergic , carbohydrate metabolism , nuclear magnetic resonance , isoflurane , nuclear magnetic resonance spectroscopy , metabolism , glutamate receptor , biochemistry , biology , medicine , magnetic resonance imaging , amino acid , receptor , radiology , microbiology and biotechnology , physics , organic chemistry
The application of in vivo 13 C MR spectroscopy to mouse brain models is potentially valuable for improving the understanding of cerebral carbohydrate metabolism and glutamatergic neurotransmission in various neuropathologies. However, the low sensitivity of 13 C nuclei and contaminating signals of lipids in the relatively small mouse brain make this application rather challenging. To meet these technical challenges, localized semi‐adiabatic distortionless enhanced polarization transfer (DEPT) MR spectroscopy in combination with a continuous intravenous [1,6‐ 13 C 2 ] glucose infusion was implemented to detect glucose metabolism in isoflurane‐anesthetized mice at 7T. The signal enhancement and high spectral resolution obtained in these experiments enabled the separate determination of 13 C label incorporation into as much as 13 metabolites from a 175 μL volume. Signal increases of glucose (C6), glutamine (C3, C4), and glutamate (C3, C4) were determined with a time resolution of 8.6 min. This study demonstrates an optimized MR method for the application of in vivo 13 C MRS in mouse brain. Magn Reson Med, 2008. © 2008 Wiley‐Liss, Inc.