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In vivo myocardial infarct area at risk assessment in the rat using manganese enhanced magnetic resonance imaging (MEMRI) at 1.5T
Author(s) -
Daire J.L.,
Hyacinthe J.N.,
Tatar I.,
MontetAbou K.,
Ivancevic M.K.,
Masterson K.,
JorgeCosta M.,
Morel D.R.,
Vallée J.P.
Publication year - 2008
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21493
Subject(s) - coronary occlusion , ventricle , occlusion , medicine , magnetic resonance imaging , ejection fraction , cardiology , myocardial infarction , nuclear medicine , radiology , heart failure
The aim of this study was to measure the myocardial area at risk in rat, using MRI and manganese injection during a coronary occlusion/reperfusion model at 1.5T. A sequential protocol with occlusion and MnCl 2 injection immediately followed by MRI was used with the assumption that MnCl 2 ‐induced contrast persistence is enough to accurately image the area at risk 90 min after occlusion. A total of 15 adult rats underwent a single 30‐min episode of coronary occlusion followed by reperfusion. MnCl 2 was injected (25 μmol/kg) at the beginning of the occlusion for 11 rats (group 1) and 6 h after reperfusion for four animals (group 2). A deficit of signal enhancement was observed in all rats. Hypoenhancement area in group 1 was correlated to the area at risk delineated by methylene blue (r = 0.96, P < 0.0001) whereas in group 2 it was correlated to the infarct area given by triphenyltetrazolium chloride (TTC) solution (r = 0.98, P = 0.003). The area at risk size was significantly correlated with left ventricle ejection fraction (LVEF), end‐systolic volume and anterolateral wall thickening. This work demonstrates that hypoenhanced zone obtained after manganese injection during occlusion represents the area at risk and not only the infarct zone. Magn Reson Med 59:1422–1430, 2008. © 2008 Wiley‐Liss, Inc.