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Hyperpolarized C‐13 spectroscopic imaging of the TRAMP mouse at 3T—Initial experience
Author(s) -
Chen Albert P.,
Albers Mark J.,
Cunningham Charles H.,
Kohler Susan J.,
Yen YiFen,
Hurd Ralph E.,
Tropp James,
Bok Robert,
Pauly John M.,
Nelson Sarah J.,
Kurhanewicz John,
Vigneron Daniel B.
Publication year - 2007
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21256
Subject(s) - tramp , in vivo , prostate cancer , nuclear magnetic resonance , chemistry , pulse sequence , prostate , magnetic resonance spectroscopic imaging , magnetic resonance imaging , cancer research , nuclear medicine , cancer , medicine , biology , physics , microbiology and biotechnology , radiology
Abstract The transgenic adenocarcinoma of mouse prostate (TRAMP) mouse is a well‐studied murine model of prostate cancer with histopathology and disease progression that mimic the human disease. To investigate differences in cellular bioenergetics between normal prostate epithelial cells and prostate tumor cells, in vivo MR spectroscopic (MRS) studies with non‐proton nuclei, such as 13 C, in the TRAMP model would be extremely useful. The recent development of a method for retaining dynamic nuclear polarization (DNP) in solution permits high signal‐to‐noise ratio (SNR) 13 C MRI or MRSI data to be obtained following injection of a hyperpolarized 13 C agent. In this transgenic mouse study, this method was applied using a double spin‐echo (DSE) pulse sequence with a small‐tip‐angle excitation RF pulse, hyperbolic‐secant refocusing pulses, and a flyback echo‐planar readout trajectory for fast (10–14 s) MRSI of 13 C pyruvate (pyr) and its metabolic products at 0.135 cm 3 nominal spatial resolution. Elevated 13 C lactate (lac) was observed in both primary and metastatic tumors, demonstrating the feasibility of studying cellular bioenergetics in vivo with DNP hyperpolarized 13 C MRSI. Magn Reson Med, 2007. © 2007 Wiley‐Liss, Inc.