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Enhanced T 2 contrast for MR histology of the mouse brain
Author(s) -
Sharief Anjum Ali,
Johnson G. Allan
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.21026
Subject(s) - contrast (vision) , nuclear magnetic resonance , susceptibility weighted imaging , fixative , sequence (biology) , diffusion imaging , anatomy , chemistry , physics , magnetic resonance imaging , diffusion mri , biology , optics , medicine , biochemistry , cytoplasm , radiology
A 3D Carr‐Purcell‐Meiboom‐Gill (CPMG) sequence was implemented to obtain enhanced T 2 contrast in actively stained (perfusion with fixative and contrast agent) mouse brains at 9.4 T. Short interecho spacing was used to minimize diffusion and susceptibility losses. The sequence produced 16 3D volumes with an interecho spacing of 7 ms for isotropic 43‐μ‐resolution images of the mouse brains in a scan time of 4 hr. To enhance the signal‐to‐noise ratio (SNR) and contrast, the multiecho frequency domain image contrast (MEFIC) method was applied, resulting in a composite image with T 2 ‐weighted contrast. The high SNR and contrast thus achieved revealed aspects of mouse brain morphology, such as multiple cortical layers, groups of thalamic nuclei, layers of the inferior and superior colliculus, and molecular and granular layers of the cerebellum, with a high degree of definition and contrast that was not previously achieved in T 2 ‐weighted acquisitions at high fields. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.