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In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy
Author(s) -
Smirnov Pierre,
Lavergne Elise,
Gazeau Florence,
Lewin Maïté,
Boissonnas Alexandre,
Doan BichThuy,
Gillet Brigitte,
Combadière Christophe,
Combadière Béhazine,
Clément Olivier
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20996
Subject(s) - in vivo , spleen , ex vivo , splenocyte , cell , cancer research , lymphocyte , chemistry , ferumoxytol , pathology , medicine , magnetic resonance imaging , immunology , biology , biochemistry , microbiology and biotechnology , radiology
The aim of this study was to demonstrate the feasibility of in vivo cell tracking to monitor anticancer cell therapy by means of a high‐resolution noninvasive MRI method. Ovalbumin‐specific splenocytes (OT‐1) labeled with anionic γ‐Fe 2 O 3 superparamagnetic iron oxide (SPIO) nanoparticles were adoptively transferred into C57BL/6 mice with growing ovalbumin‐expressing tumors. OT‐1 cells were tracked in vivo by 7 T MRI 24, 48, and 72 hr after they were injected. The results showed significant negative enhancement of the spleen at 24 hr, and of the tumor at 48 and 72 hr, after labeled cell injection. This suggests that the lymphocytes initially homed toward the spleen and were then recruited by the tumor. The presence of labeled cells was confirmed in ex vivo by 9.4 T microimaging of tumors and magnetic sorting of spleen cells. These results confirm that MR tracking of lymphocytes is feasible in vivo. This high‐resolution imaging method could be used to improve the monitoring of immune cell therapy. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.