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DTI‐based assessment of ischemia‐reperfusion in mouse skeletal muscle
Author(s) -
Heemskerk Anneriet M.,
Drost Maarten R.,
van Bochove Glenda S.,
van Oosterhout Matthijs F.M.,
Nicolay Klaas,
Strijkers Gustav J.
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20953
Subject(s) - diffusion mri , ischemia , fractional anisotropy , skeletal muscle , occlusion , effective diffusion coefficient , medicine , anatomy , magnetic resonance imaging , radiology
Diffusion tensor imaging (DTI) is frequently applied to characterize the microscopic geometrical properties of tissue. To establish whether and how diffusion MRI responds to transient ischemia of skeletal muscle, we studied the effects of ischemia and reperfusion using DTI and T 2 ‐weighted MRI before and during ischemia and up to 24 hr after reperfusion. Ischemia was induced by 50 min of hindlimb occlusion with or without dorsal flexor stimulation. During ischemia the apparent diffusion coefficient (ADC) tended to decrease (up to 15%), whereas the fractional anisotropy (FA) and T 2 showed a varied response depending on the protocol and muscle type. During reperfusion the ADC and T 2 initially increased and subsequently renormalized for the occlusion protocol. For the occlusion plus stimulation (OS) protocol, the FA was decreased by 13% and the ADC and T 2 were increased by 20% and 57%, respectively, after 24 hr in the stimulated muscle complex. In the latter tissue the three DTI eigenvalues gradually increased upon reperfusion. The smallest eigenvalue (λ 3 ) showed the largest relative increase. Changes in DTI indices in the reperfusion phases followed a similar time course as the changes in T 2 . The changes in MR indices after 24 hr correlated with the tissue damage quantified with histology. The highest correlation was observed for λ 3 (R 2 = 0.81). This study shows that DTI can be used to assess ischemia‐induced damage to skeletal muscle. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.