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Liposome‐enhanced MRI of neointimal lesions in the ApoE‐KO mouse
Author(s) -
Mulder Willem J.M.,
Douma Kim,
Koning Gerben A.,
van Zandvoort Marc A.,
Lutgens Esther,
Daemen Mat J.,
Nicolay Klaas,
Strijkers Gustav J.
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20883
Subject(s) - liposome , magnetic resonance imaging , medicine , artery , apolipoprotein e , nuclear medicine , chemistry , pathology , radiology , biochemistry , disease
Abstract Conventional high‐resolution MRI is capable of detecting lipid‐rich atherosclerotic plaques in both human atherosclerosis and animal models of atherosclerosis. In this study we induced neointimal lesions in ApoE‐KO mice by placing a constrictive collar around the right carotid artery. The model was imaged with conventional multispectral MRI, and the thickened wall could not be distinguished from surrounding tissue. We then tested paramagnetic liposomes (mean size = 90 nm) for their ability to improve MRI visualization of induced thickening, using Gd‐DTPA as a control. T 1 ‐weighted ( T 1 ‐w), black‐blood MRI of the neck area of the mice was performed before and 15 min, 45 min, and 24 hr after intravenous injection of either paramagnetic liposomes or Gd‐DTPA. The collared vessel wall of mice that were injected with liposomes showed a pronounced signal enhancement of ∼100% immediately after injection, which was sustained largely until 24 hr postinjection. In contrast, the vessel wall of all controls (left carotid artery and animals injected with Gd‐DTPA) did not show significant contrast enhancement at those time points. This study demonstrates that intimal thickening in ApoE‐KO mice can be effectively detected by contrast‐enhanced (CE)‐MRI upon injection of paramagnetic liposomes. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.