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Quantification of vitamin C in the rat brain in vivo using short echo‐time 1 H MRS
Author(s) -
Terpstra Melissa,
Tkác̆ Ivan,
Rao Raghavendra,
Gruetter Rolf
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20854
Subject(s) - chemistry , ascorbic acid , in vivo , glutathione , glutamine , vitamin c , nuclear magnetic resonance , biochemistry , biology , amino acid , food science , microbiology and biotechnology , enzyme , physics
Both ultrashort echo‐time STEAM and MEGA‐PRESS‐edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, glutamate, glutathione, and macromolecules, reliable quantification of ascorbate (Cramer‐Rao lower bounds < 0.2 μmol/g) by LCModel analysis of STEAM (TE = 2 ms) spectra was possible at 9.4 T. Ascorbate concentrations quantified from the STEAM spectra were in very good agreement with concentrations calculated from fully resolved ascorbate resonances in MEGA‐PRESS‐edited spectra measured from identical volumes of interest. Ascorbate concentrations measured using STEAM decreased with increasing postnatal rat age, in agreement with published brain ascorbate concentrations measured in vitro using high‐performance liquid chromatography (HPLC). Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.