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Localized in vivo isotropic‐anisotropic correlation 1 H NMR spectroscopy using ultraslow magic angle spinning
Author(s) -
Wind Robert A.,
Hu Jian Z.,
Majors Paul D.
Publication year - 2006
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20740
Subject(s) - isotropy , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , pulse sequence , magic angle spinning , spectroscopy , magic angle , spinning , anisotropy , in vivo , magic (telescope) , spectral line , chemistry , materials science , physics , optics , biology , microbiology and biotechnology , quantum mechanics , astronomy , polymer chemistry
In a previous work (1), the susceptibility broadening in the 1 H NMR metabolite spectrum obtained in a live mouse was separated from the isotropic information, which significantly increased the spectral resolution. This was achieved using ultraslow magic angle spinning (MAS) of the animal combined with a modified phase‐corrected magic angle turning (PHORMAT) pulse sequence. However, PHORMAT cannot be used for spatially selective spectroscopy. This article introduces a modified sequence called localized magic angle turning (LOCMAT) that makes this possible. Proton LOCMAT spectra were obtained from the liver and heart of a live mouse while the animal was spun at a speed of 4 Hz in a 2 Tesla field. It was found that even in this relatively low field, LOCMAT provided isotropic line widths that were a factor of 4–10 times smaller than those obtained in a stationary animal. Furthermore, the susceptibility broadening of the heart metabolites showed unusual features that are not observed in dead animals. The limitations of LOCMAT and possible ways to improve the technique are discussed. It is concluded that in vivo LOCMAT can significantly enhance the utility of NMR spectroscopy for biomedical research. Magn Reson Med, 2006. Published 2005 Wiley‐Liss, Inc.