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Monitoring T‐lymphocyte trafficking in tumors undergoing immune rejection
Author(s) -
Hu DeEn,
Kettunen Mikko I.,
Brindle Kevin M.
Publication year - 2005
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20717
Subject(s) - immune system , infiltration (hvac) , immunohistochemistry , pathology , staining , t lymphocyte , intracellular , lymphocyte , ovalbumin , chemistry , cancer research , microbiology and biotechnology , biology , medicine , immunology , materials science , biochemistry , composite material
Abstract Activated T cells, isolated from animals that had rejected a tumor (E.G7‐OVA) expressing chicken ovalbumin, were labeled with citrated superparamagnetic iron oxide nanoparticles at an intracellular iron concentration of up to 0.5 pg/cell. Injection of these labeled T cells into animals bearing E.G7‐OVA tumors undergoing immune rejection resulted in tumor infiltration of these cells, which was detectable as a heterogeneous decrease in intensity in T 2 ‐weighted MR images. T‐cell infiltration was confirmed by immunohistochemical staining of tumor sections obtained postmortem and was shown to colocalize with iron that had been stained using Prussian blue. Tumor rejection was correlated with the uptake of labeled T cells, since the infiltration of labeled T cells was only observed in those tumors that went on to regress. This technique should assist in the elucidation of those factors that are important in mediating tumor immune rejection. Magn Reson Med, 2005. © 2005 Wiley‐Liss, Inc.