Premium
Absolute oxygen tension (pO 2 ) in murine fatty and muscle tissue as determined by EPR
Author(s) -
Matsumoto Atsuko,
Matsumoto Shingo,
Sowers Anastasia L.,
Koscielniak Janusz W.,
Trigg Nancy J.,
Kuppusamy Periannan,
Mitchell James B.,
Subramanian Sankaran,
Krishna Murali C.,
Matsumoto Kenichiro
Publication year - 2005
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20714
Subject(s) - electron paramagnetic resonance , chemistry , muscle tissue , oxygen , nuclear magnetic resonance , anatomy , medicine , physics , organic chemistry
Abstract The absolute partial pressure of oxygen (pO 2 ) in the mammary gland pad and femoral muscle of female mice was measured using EPR oximetry at 700 MHz. A small quantity of lithium phthalocyanine (LiPc) crystals was implanted in both mammary and femoral muscle tissue of female C3H mice. Subsequent EPR measurements were carried out 1–30 days after implantation with or without control of core body temperature. The pO 2 values in the tissue became stable 2 weeks after implantation of LiPc crystals. The pO 2 level was found to be higher in the femoral muscle than in the mammary tissue. However, the pO 2 values showed a strong dependence on the core body temperature of the mice. The pO 2 values were responsive to carbogen (95% O 2 , 5% CO 2 ) breathing even 44–58 days after the implantation of LiPc. The LiPc linewidth was also sensitive to changes in the blood supply even 60 days after implantation of the crystals. This study further validates the use of LiPc crystals and EPR oximetry for long‐term non‐invasive assessment of pO 2 levels in tissues, underscores the importance of maintaining normal body core temperature during the measurements, and demonstrates that mammary tissue functions at a lower pO 2 level than muscle in female C3H mice. Magn Reson Med, 2005. Published 2005 Wiley‐Liss, Inc.