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Comparison of [3,4‐ 13 C 2 ]glucose to [6,6‐ 2 H 2 ]glucose as a tracer for glucose turnover by nuclear magnetic resonance
Author(s) -
Jin Eunsook S.,
Jones John G.,
Burgess Shawn C.,
Merritt Matthew E.,
Dean Sherry A.,
Malloy Craig R.
Publication year - 2005
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20496
Subject(s) - tracer , chemistry , carbohydrate metabolism , d glucose , in vivo , glucose uptake , radiochemistry , endocrinology , biochemistry , insulin , medicine , biology , physics , microbiology and biotechnology , nuclear physics
A recently introduced tracer, [3,4‐ 13 C 2 ]glucose, was compared to the widely used tracer, [6,6‐ 2 H 2 ]glucose, for measurement of whole‐body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4‐ 13 C 2 ]glucose, [6,6‐ 2 H 2 ]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into monoacetone glucose for analysis by 13 C NMR (for [3,4‐ 13 C 2 ]glucose) or 1 H and 2 H NMR (for [6,6‐ 2 H 2 ]glucose). The values of GP measured during infusion of each single tracer were not significantly different. In rats infused with both tracers simultaneously, GP was identical as reported by each tracer, 42 ± 4 μmol/kg/min. Since 2 H and 13 C enrichment in glucose is typically much less than 2% for in vivo studies, [3,4‐ 13 C 2 ]glucose does not interfere with measurements of 13 C or 2 H enrichment patterns and therefore is valuable when multiple metabolic pathways are being evaluated simultaneously. Magn Reson Med 53:1479–1483, 2005. © 2005 Wiley‐Liss, Inc.