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In vivo detection of cortical GABA turnover from intravenously infused [1‐ 13 C]D‐glucose
Author(s) -
Yang Jehoon,
Li Charles Q.,
Shen Jun
Publication year - 2005
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20473
Subject(s) - glutamine , in vivo , chemistry , glutamate receptor , biochemistry , amino acid , biology , receptor , microbiology and biotechnology
In this study [2‐ 13 C] γ‐aminobutyric acid (GABA) was spectrally resolved in vivo and detected simultaneously with [4‐ 13 C]glutamate (Glu) and [4‐ 13 C]glutamine (Gln) in the proton spectra obtained from a localized 40 μL voxel in rat neocortex with the use of an adiabatic 1 H‐observed, 13 C‐edited (POCE) spectroscopy method and an 89‐mm‐bore vertical 11.7 Tesla microimager. The time‐resolved kinetics of 13 C label incorporation from intravenously infused [1‐ 13 C]glucose into [4‐ 13 C]Glu, [4‐ 13 C]Gln, and [2‐ 13 C]GABA were measured after acute administration of gabaculine, a potent and specific inhibitor of GABA‐transaminase. In contrast to previous observations of a rapid turnover of [2‐ 13 C]GABA from [1‐ 13 C]glucose in intact rat brain, the rate of 13 C incorporation from [1‐ 13 C]glucose into [2‐ 13 C]GABA in the gabaculine‐treated rats was found to be significantly reduced as a result of the blockade of the GABA shunt. Magn Reson Med 53:1258–1267, 2005. Published 2005 Wiley‐Liss, Inc.