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NMR analysis of neutrophil activation in sputum samples from patients with cystic fibrosis
Author(s) -
Saude Erik J.,
Lacy Paige,
MusatMarcu Sorin,
Mayes Damon C.,
Bagu John,
Man S.F. Paul,
Sykes Brian D.,
Moqbel Redwan
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20242
Subject(s) - sputum , cystic fibrosis , eosinophil , chemistry , stimulation , in vitro , neutrophil extracellular traps , tyrosine , respiratory system , immunology , pathology , medicine , inflammation , biochemistry , asthma , tuberculosis
Disorders of the respiratory system, such as cystic fibrosis (CF), involve the infiltration and activation of airway inflammatory cells, including neutrophils. This leads to the secretion of peroxidases, which react further with substrates in solution to produce oxidative metabolites, such as 3‐chlorotyrosine. Elevated levels of modified tyrosine residues in the airways of patients with CF may be detectable by nuclear magnetic resonance (NMR) in correlation with inflammatory cell influx. In this study, high‐resolution (500 MHz) 1 H NMR was used to analyze the production of modified tyrosine residues resulting from in vitro stimulation of peripheral blood eosinophils and neutrophils, as well as in sputum samples from control subjects and patients with CF. Following in vitro stimulation, purified peripheral blood neutrophils generated 3‐chlorotyrosine, while eosinophils produced predominantly 3‐bromotyrosine and 3,5‐dibromotyrosine. Chlorinated and brominated tyrosine residues were detected in sputum samples from patients with CF ( N = 7), but were not detected in the control group ( N = 9). Neutrophil counts in CF sputum correlated strongly with the presence of 3‐chlorotyrosine ( r 2 = 0.869). Our findings indicate that neutrophil and eosinophil activation in CF is detectable by NMR. NMR may be a useful tool for the detection of biological markers of inflammatory processes in patient airways. Magn Reson Med 52:807–814, 2004. © 2004 Wiley‐Liss, Inc.