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Effects of physiologic challenge on the ADC of intracellular water in the Xenopus oocyte
Author(s) -
Sehy Jonathan V.,
Zhao Lin,
Xu Junqian,
Rayala Heidi J.,
Ackerman Joseph J.H.,
Neil Jeffrey J.
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20132
Subject(s) - intracellular , oocyte , chemistry , biophysics , xenopus , nocodazole , microtubule , intracellular ph , microbiology and biotechnology , biochemistry , biology , cell , cytoskeleton , embryo , gene
Abstract The biophysical determinants of the intracellular water apparent diffusion coefficient (ADC) in mammalian tissues are poorly understood. Model systems that are more amenable to physical measurements may provide insights into the behavior of more complex systems. Toward that end, we used MRI to evaluate the effects of altered microtubule concentration, nuclear breakdown, and ATP depletion on intracellular water ADC in the Xenopus oocyte. Water ADC did not change in response to polymerization of microtubules with taxol or depolymerization with nocodazole. Water ADC did not change following the breakdown of the nucleus in healthy cells. Short‐term depletion of ATP (∼20% of normal levels following 4 hr of exposure to sodium azide and 2‐deoxy‐D‐glucose) was not associated with a change in intracellular ADC. Long‐term depletion of ATP (∼20% of normal levels following 2 days of exposure to antimycin A) was associated with a significant decrease in intracellular water ADC. These findings suggest that intracellular water diffusion in oocytes is not dependent on the state of microtubule polymerization or short‐term ATP depletion, although long‐term ATP depletion is associated with changes that lead to a decrease in intracellular water ADC. Magn Reson Med 52:239–247, 2004. © 2004 Wiley‐Liss, Inc.