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Lactate isotopomer analysis by 1 H NMR spectroscopy: Consideration of long‐range nuclear spin–spin interactions
Author(s) -
Lloyd Steven G.,
Zeng Huadong,
Wang PeiPei,
Chatham John C.
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20075
Subject(s) - isotopomers , nuclear magnetic resonance spectroscopy , chemistry , metabolite , spectroscopy , isotope , nuclear magnetic resonance , analytical chemistry (journal) , biochemistry , stereochemistry , chromatography , physics , molecule , organic chemistry , quantum mechanics
Lactate is a key metabolite and its rates of cellular uptake and release, its production rates from glucose and glycogen, and its interconversion rate with pyruvate are important determinants of cellular energy production. If lactate precursors such as pyruvate and glucose are labeled appropriately with 13 C, 1 H NMR spectroscopy provides a means of quantifying lactate production from each source and allows measurement of all these rates within a single experiment. However, due to the multiplicities of the resonance lines (from nuclear spin–spin couplings) in lactate 13 C isotopomers, the 1 H NMR spectra were found to be more complex than expected, requiring determination of all spin–spin interactions in this anion. All such values were determined for lactate and its precursor pyruvate. The method was then applied to simultaneously measure the rates of exogenous lactate uptake and rates of release of glucose‐, glycogen‐, and pyruvate‐derived lactate in the isolated perfused rat heart. Magn Reson Med 51:1279–1282, 2004. © 2004 Wiley‐Liss, Inc.