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Glucose production pathways by 2 H and 13 C NMR in patients with HIV‐associated lipoatrophy
Author(s) -
Weis Brian C.,
Margolis David,
Burgess Shawn C.,
Merritt Matthew E.,
Wise Holly,
Sherry A. Dean,
Malloy Craig R.
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.20057
Subject(s) - lipoatrophy , chemistry , human immunodeficiency virus (hiv) , nuclear magnetic resonance , radiochemistry , medicine , physics , antiretroviral therapy , virology , viral load
Patients with HIV taking protease inhibitors were selected for the presence (five subjects) or absence (five subjects) of lipoatrophy. Following an overnight fast, subjects were given oral 2 H 2 O in divided doses (5 mL/kg body water), [U‐ 13 C 3 ] propionate (10 mg/kg), and acetaminophen (1000 mg). Glucose (from plasma) or acetaminophen glucuronide (from urine) were converted to monoacetone glucose for 2 H NMR and 13 C NMR analysis. The fraction of plasma glucose derived from gluconeogenesis was not significantly different between groups. However, flux from glycerol into gluconeogenesis relative to glucose production was increased from 0.20 ± 0.13 among subjects without lipoatrophy to 0.42 ± 0.12 ( P < 0.05) among subjects with lipoatrophy, and the TCA cycle contribution was reduced. Lipoatrophy was associated with an abnormal profile of glucose production as assessed by 13 C and 2 H NMR of plasma and urine. Magn Reson Med 51:649–654, 2004. Published 2004 Wiley‐Liss, Inc.