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Enhancement of nitroxide‐reducing activity in rats after chronic administration of vitamin E, vitamin C, and idebenone examined by an in Vivo electron spin resonance technique
Author(s) -
Matsumoto Seiji,
Mori Norio,
Tsuchihashi Nobuaki,
Ogata Tateaki,
Lin Yijing,
Yokoyama Hidekatsu,
Ishida ShinIchi
Publication year - 1998
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910400219
Subject(s) - idebenone , in vivo , antioxidant , vitamin c , electron paramagnetic resonance , chemistry , vitamin , vitamin e , nitroxide mediated radical polymerization , spin label , endocrinology , medicine , pharmacology , biochemistry , nuclear magnetic resonance , biology , physics , microbiology and biotechnology , radical polymerization , organic chemistry , copolymer , polymer
Rats were given vitamin E (vit‐E), idebenone (ID), or vitamin (C wit‐C) in their food for 2 or 4 weeks. After feeding, the ability of rats to reduce 4‐hydroxy‐2,2,6,6‐tetramethylpiperidine‐l‐oxyl (Tempol) in terms of the half‐life of Ternpol was examined as a specific marker. Ternpol was repeatedly injected intravenously, and its half‐life was serially evaluated by an in vivo electron spin resonance (ESR) technique. The radical‐reducing ability in rats was enhanced differently by Vit‐E, ID, and Vit‐C, i.e., slow onset of the ability after Vit‐E and ID (lipid‐soluble antioxidants) and fast onset after Vit‐C (a water‐soluble antioxidant).