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Detecting natural abundance carbon signal of NAA metabolite within 12‐cm 3 localized volume of human brain using 1 H‐{ 13 C} NMR spectroscopy
Author(s) -
Chen Wei,
Adriany Gregor,
Zhu XiaoHong,
Gruetter Rolf,
Ugurbil Kamil
Publication year - 1998
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910400203
Subject(s) - metabolite , nuclear magnetic resonance spectroscopy , chemistry , spectroscopy , human brain , nuclear magnetic resonance , biochemistry , biology , stereochemistry , physics , quantum mechanics , neuroscience
NMR spectroscopy has been applied extensively to study metabolism noninvasively in the human brain and other tissues. However, it usually suffers from poor signal‐to‐noise ratio due to low NMR sensitivity and low metabolite concentrations. In this study, the technique of proton‐observe‐carbon‐edited (POCE) NMR spectroscopy combined with a single‐shot localization sequence was used to detect the natural abundance carbon signal of the amino acid N‐acetyl aspartate from a 12‐cm 3 localized volume in the occipital lobe of humans at 4 T. The results suggest that NMR spectroscopy is sensitive enough to detect signals from low concentration metabolites (<60 nmol/g) from small volumes in the human brain within several minutes of data acquisition. This reveals that in vivo NMR spectroscopy is a promising technique for detecting small metabolite changes and low traces of 13 C isotopic labeling for dynamic metabolism studies aimed at investigating physiological and pathological questions.