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Simultaneous intracellular and extracelular p H measurement in the heart by 19 F NMR of 6‐fluoropyridoxol
Author(s) -
Hunjan Sandeep,
Mason Ralph P.,
Mehta Vimal D.,
Kulkarni Padmakar V.,
Aravind Subramanian,
Arora Veeva,
Antich Peter P.
Publication year - 1998
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910390407
Subject(s) - chemistry , physics , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy
6‐Fluoropyridoxol (6‐FPOL) was evaluated as a simultaneous indicator of intracellular and extracellular pH and, hence, pH gradient in perfused rat hearts. After infusion, 19 F NMR spectra rapidly showed two well‐resolved peaks assigned to the intracellular and extracellular compartments, and pH was calculated on the basis of chemical shift with respect to a sodium trifluoroacetate standard. To demonstrate use of this molecule, dynamic changes in myocardial pH were assessed with a time resolution of 2 min during respiratory and metabolic alkalosis or acidosis and ischemia. For a typical heart, intracellular pH (pHi) = 7.14 ± 0.01 and extracellular pH (pHe) = 7.52 ± 0.02. In response to metabolic alkalosis, pHi remained relatively constant and the pH gradient increased. In contrast, respiratory challenge caused a significant increase in pHi. Independent measurements using pH electrodes and 31 P NMR confirmed validity of the 19 F NMR results.