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Determination of the intracellular sodium concentration in perfused mouse liver by 31 P and 23 Na magnetic resonance spectroscopy
Author(s) -
Colet JeanMarie,
Makos Janice D.,
Malloy Craig R.,
Sherry A. Dean
Publication year - 1998
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910390122
Subject(s) - intracellular , extracellular , sodium , chemistry , nuclear magnetic resonance spectroscopy , intracellular ph , spectroscopy , isotopes of sodium , nuclear magnetic resonance , analytical chemistry (journal) , resonance (particle physics) , extracellular fluid , nuclear chemistry , chromatography , biochemistry , stereochemistry , physics , organic chemistry , quantum mechanics , particle physics
A combination of 31 P and 23 Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na] IC in the isolated and perfused mouse liver. The 31 P resonances of dimethyl methylphosphonate and LaDOTP 5− , markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 ± 0.3 g, the intracellular liver volume as measured by 31 P NMR averaged 1.2 ± 0.2 ml. The amount of intracellular sodium was measured from the baseline‐resolved intracellular 23 Na resonance during perfusion of the shift reagent, TmDOTP 5− . These two measurements resulted in an NMR‐determined value for [Na] IC of 29.0 ± 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS‐determined value for [Na] Ic of 32.1 ± 7.4 mM . These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23 Na NMR spectroscopy.