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Evaluation of triple quantum‐filtered 23 Na NMR spectroscopy in the in situ rat liver
Author(s) -
Seshan Viswanathan,
Sherry A. Dean,
Bansal Navin
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380519
Subject(s) - chemistry , signal (programming language) , nuclear magnetic resonance , spectroscopy , nuclear magnetic resonance spectroscopy , reagent , sodium , isotopes of sodium , analytical chemistry (journal) , physics , chromatography , organic chemistry , quantum mechanics , computer science , programming language
Triple quantum (TQ)‐filtered 23 Na NMR spectroscopy and the shift reagent, TmDOTP 5‐ , have been used to evaluate the contributions of intra‐ (Na i + ) and extracellular (Na e + ) sodium to the TQ‐filtered signal in the rat liver, in situ. Na e + contributed significantly to the total TQ‐filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ‐filtered Na i + signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na i + increased by 90%. The constancy of the TQ‐filtered Na e + signal indicates that changes in total TQ‐filtered 23 Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ‐filtered Na i + signal intensity. The large percent increase in the TQ‐filtered Na i + signal as compared to the SQ signal suggests that the fraction of Na i + interacting with macromolecules increases after death.