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Study of the metabolism of choline and phosphatidylcholine in tumors in vivo using phosphonium‐choline
Author(s) -
Street James C.,
Szwergold Benjamin S.,
Matei Cornelia,
Kappler Francis,
Mahmood Umar,
Brown Truman R.,
Koutcher Jason A.
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380513
Subject(s) - phosphocholine , choline , phosphatidylcholine , betaine , phosphonium , in vivo , phosphorylcholine , chemistry , metabolism , phospholipid , choline kinase , biochemistry , biology , organic chemistry , microbiology and biotechnology , membrane
The results of an initial study on the feasibility of using the phosphonium analog of choline to follow the metabolism of phosphatidylcholine in tumors in vivo using 31 P NMR are reported. C3H/He mice bearing a mammary carcinoma tumor on the foot pad were fed a choline‐free diet supplemented with the phosphonium analog of choline. Metabolites of this compound, including the phosphonium analogs of phosphatidylcholine, phosphocholine, glycerophosphocholine, and betaine were observed noninvasively in vivo in tumors by 31 P NMR after 2–3 weeks of feeding. Clearance of these phosphonium‐labeled metabolites from tumors was measured after a change to a choline‐containing diet. Significant decreases were seen in the levels of the analogs of betaine ( P < 0.003) and phosphatidylcholine ( P < 0.004) by Day 4. A significant increase in the level of authentic phosphocholine ( P < 0.003) occurred over the same time period.