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Low visibility of lactate in excised rat muscle using double quantum proton spectroscopy
Author(s) -
Jouvensal Laurence,
Carlier Pierre G.,
Bloch Gilles
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380505
Subject(s) - visibility , proton , spectroscopy , nuclear magnetic resonance , chemistry , nuclear magnetic resonance spectroscopy , physics , biophysics , biology , optics , nuclear physics , quantum mechanics
Lactate NMR visibility was investigated in excised rat muscle at 3 T by comparing the concentration determined in situ by double quantum (DQ) proton spectroscopy (150 ms effective echo time) to the concentration measured in vitro from perchloric acid extracts of the same muscle samples. After 1‐2 h of ischemia, lactate NMR visibility was 32 ± 3% (±SE, n = 9), and was only 21 ± 1% ( n = 6) after 10–12 h. Muscle lactate T 2 was 140 ± 11 ms and 184 ± 6 ms, respectively. All potential mechanisms of DQ lactate signal attenuation ( B 0 , and B 1 inhomogeneity, DQ transverse relaxation, diffusion) were examined, and accounted for when necessary. A significant increase in lactate NMR visibility was demonstrated using a shorter effective echo time (79 ms) DQ editing sequence. These results are interpreted as reflecting muscle lactate compartmentation between a long T 2 pool predominantly detected by DQ spectroscopy, and a short T 2 pool virtually invisible with longer echo time NMR techniques.