Premium
Reduction of carcinogenic chromium(vi) on the skin of living rats
Author(s) -
Liu Ke Jian,
Mäder Karsten,
Shi Xianglin,
Swartz Harold M.
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380403
Subject(s) - electron paramagnetic resonance , chromium , chromate conversion coating , chemistry , electron paramagnetic resonance spectroscopy , in vivo , aqueous solution , carcinogen , paramagnetism , spectroscopy , nuclear magnetic resonance , nuclear chemistry , photochemistry , biophysics , biochemistry , biology , organic chemistry , physics , microbiology and biotechnology , quantum mechanics
The reduction of chromate (Cr(VI)) on the skin of living rats was investigated using the technique of in vivo electron paramagnetic resonance (EPR) spectroscopy with a surface coil resonator. Topical application of aqueous solution of Cr(VI) on the skin of rats generated the transient species, Cr(V). Partial removal of the stratum corneum increased the rates of formation and decay of Cr(V), as well as Cr(V) signal intensity. The results demonstrate that skin represents one route for chromium to enter into animals and humans. The generation of reactive Cr(V) species in the reduction of Cr(VI) by the skin may play an important role in the mechanism of Cr(VI)‐induced skin cancer. These findings indicate the potential for in vivo EPR spectroscopy for studying the metabolism of paramagnetic reactive species in chemical and biochemical reactions occurring in/on the skin of both small and large animals, and possibly humans.