Premium
Quantitative measurements of regional cerebral blood volume using MRI in rats: Effects of arterial carbon dioxide tension and mannitol
Author(s) -
Lin Weili,
Paczynski Richard P.,
Kuppusamy Karthikeyan,
Hsu Chung Y.,
Haacke E. Mark
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380311
Subject(s) - mannitol , tonicity , blood volume , carbon dioxide , contrast (vision) , chemistry , voxel , anesthesia , nuclear medicine , medicine , radiology , computer science , organic chemistry , artificial intelligence
A three‐dimensional (3D) T 1 ‐weighted sequence was used to acquire high spatial resolution whole brain images in rats before and after the injection of an intravascular contrast agent. These T 1 ‐weighted images were used to estimate regional cerebral blood volume (rCBV) as a percentage of blood volume in each voxel. Ventilation was manipulated to investigate the effects of altered arterial carbon dioxide tension (PaCO 2 ) on rCBV. In addition, different doses of a hypertonic mannitol solution were used to investigate the sensitivity of the proposed method in a serial monitoring paradigm. An rCBV of 2.40% ± 0.34% was obtained before any physiological manipulation, in good agreement with literature values using alternative techniques. Using this method, it was found that there exists a linear relationship between PaCO 2 and rCBV (R 2 = 0.77) and that rCBV increased in a dose and time dependent fashion in mannitol‐treated rats. High signal‐to‐noise was available due to the substantial increase in blood signal from the intravascular contrast agent.