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Direct observation of resolved intracellular and extracellular water signals in intact human red blood cells using 1 H MAS NMR spectroscopy
Author(s) -
Humpfer Eberhard,
Spraul Manfred,
Nicholls Andrew W.,
Nicholson Jeremy K.,
Lindon John C.
Publication year - 1997
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910380224
Subject(s) - extracellular , intracellular , nuclear magnetic resonance spectroscopy , spectroscopy , chemistry , magic angle spinning , nuclear magnetic resonance , analytical chemistry (journal) , relaxation (psychology) , spectral line , nmr spectra database , intracellular ph , laser linewidth , chromatography , biochemistry , stereochemistry , physics , optics , biology , quantum mechanics , astronomy , neuroscience , laser
High resolution 400 MHz 1 H NMR spectra of red blood cell suspensions when measured using magic angle spinning (MAS) show two water resonances separated by 15 Hz. Based on addition of a paramagnetic Mn‐EDTA complex, measurement of relaxation times and variation of extracellular H 2 O/D 2 O ratios, these have been assigned as intracellular (line‐width 17.5 Hz) and extracellular water (linewidth 4.6 Hz). This is the first direct observation of intracellular water using NMR spectroscopy and the 1 H MAS NMR spectroscopic approach offers the possibility of studying directly the compartmentation of substances in cells and kinetics of molecular transport.